![]() To date, several ELISA kits for IBD are on the market and the most popular kits use the whole virus. Regarding IBD diagnosis, the World Organization for Animal Health (OIE) recommends the use of Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of immune responses mainly to monitor vaccination programs (OIE 2018). Infectious bursal disease (IBD) occurs worldwide and its control depends mainly on accurate vaccination programs. The hypervariable region of VP2 is contained between the amino acid 204 and 344 and possesses the conformational epitopes that elicit protective immunity and the determinants responsible for the interaction with the host cell (Brandt et al. The individual expression of VP2-441, or the cleavage processing intermediate VP2-452 (452 residues), leads to the assembly of icosahedral Tâ=â1 subviral particles (SVP) of ~â23 nm formed by 20 trimers of VP2 (Coulibaly et al. When segment A is expressed in heterologous systems, multimeric particles with different architectures are spontaneously assembled (Martinez-Torrecuadrada et al. Segment B encodes for VP1, the RNA-dependent RNA polymerase. pVP2 undergoes further processing in which cellular and viral proteases and VP2 itself participate to yield mature VP2 (441 residues) and several C-terminal fragments that remain associated with the capsid (Irigoyen et al. Segment A is expressed as a polyprotein precursor that undergoes self-cleavage to release pVP2 (the precursor capsid protein of 512 residues), VP3 (the scaffolding protein) and VP4 (the protease). The viral genome is composed of two segments of dsRNA, called segment A and B. Infectious bursal disease virus (IBDV), a non-enveloped virus within the Birnaviridae family, is the etiological agent of an immunosuppressive disease that affects young chickens. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. ![]() Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry.
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